Standardization of ELISA for Detection of A vian Influenza Virus
نویسندگان
چکیده
An indirect enzyme-Iinked immunosorbent assay (ELISA) was developed for the rapid and efficient large scale screening of antibodies to avian influenza virus (AIV) infection in chicken. Antigen was a whole-purified influenza virus produced from the H9N2 subtype. Optimum dilution for goat anti-chicken conjugate to be used in the ELISA was 1: 1000, as determined by signal-to-noise ratio. The antigen concentration was 0.375J.1g of protein per weil, as determined by checkerboard titration. The sensitivity of the ELISA was compared with hemmaglutination inhibition test under field exposure. After testing of 656 field sera, the correlation coefficient for the results of two tests was significant (r=0.929, P<O.OOI). Testing 8 standard antisera of various subtypes (HI, H2, H4, H5, H6, H7, H9 and HIO) of AIV and AIV antibody positive and negative sera determined specificity of the ELISA. Antisera to ail 8-hemmaglutinin subtypes were strongly positive. 80th the sensitivity and specificity of the ELISA was compared to the other test. Thus, the ELISA was able to detect specific AIV antibodies and suitable for screening large numbers of samples in diagnostic laboratories.
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